MICROBIOLOGY DEPARTMENT

 

The Department of Microbiology came into existence in 1980, to cater to the diagnosis of infections in cancer patients. The department has grown from a simple bacteriology department to a full fledged Microbiology Department capable of diagnosing majority of opportunistic infections seen in these immunocompromised patients using the latest molecular tools.

Objectives:

Equipments:

Apart from routine microbiological equipments such as microscopes, incubators, laminar flow cabinets, centrifuges, hot air ovens and refrigerators, autoclaves; additional equipments available include, ultra low temperature freezers (-800C), CO 2 incubator, electrophoresis equipment, thermal cycler, ELISA reader, refrigerated super speed centrifuge, fluorescence microscope and inverted phase contrast microscope, water purification system, image capture and documentation facilities, lyophiliser, liquid nitrogen cylinders.

Clinical Services:

Diagnosis of microbial infections in cancer patients. The types of investigations include bacterial isolation, viral antibody and antigen detection, detecting viral, fungal and parasitic DNA. 

On an average the department receives 2799 number of samples for cultures and 23628 number of samples for serology per year

Diagnostic tests for the following Micro organisms:

·              Detection of opportunistic protozoa: Cryptosporidium, Cyclospora, Isospora,    Blastocystis, Microsporidium

Research / Contributions:

The department has been recognized for Ph.D under the Rajiv Gandhi University of Health Sciences. 

a) Main areas of Research

b) Original contributions reported:

Future plans:

FACULTY PROFILE 

Name

Dr. R.S. Jayshree

Designation

Professor and Head

Contact phone

91-80-26094073

e-mail

microjayshree@gmail.com

 

 

Name

Dr. B.G. Sumati

Designation

Associate Professor

Contact phone

91-80-26094074

e-mail

 

 

 

Name

B.R. Vijaykumar

Designation

Assistant Professor

Contact phone

91-80-26094074

e-mail

br.vijaykumar@rediffmail.com

 

RESEARCH PROJECTS COMPLETED

 

  1. “Development of Human Papilloma Virus Vaccine prototype/s-Molecular Epidemiology of HPV types prevalent in India and identification of HPV-16 L1 & E6 variants”. Funded by the Department of Biotechnology, New Delhi. India from April 2005 to March 2008.  
  2. “High-risk HPV genotypes in SIL and ASCUS in cytology based screening programme for carcinoma cervix in rural women in Karnataka.” – Funded by the Department of Biotechnology, New Delhi, India from April 2003 to March 2006.
  3. “Molecular Diagnosis of Common Opportunistic Infections in Cancer.” Funded by the Indian Council of Medical Research, New Delhi, India from 1.2.02 to 31.1.05
  4. “Molecular Epidemiology Of Cancer Of The Oral Cavity & Oropharynx & Human Papilloma Virus.” - Funded by the International Agency For Research On Cancer, Lyon, France from 1996 -1998.

 

ONGOING RESEARCH PROJECTS

 

  1. “TLR expression profile in Langerhans and Dendritic Cells in invasive cervical cancer, cervical intraepithelial neoplasia and chronic cervicitis” Funded by the Department of Biotechnology, New Delhi. India from September 2005 to March 2009. 
  2. “Regulatory T cells: a potential marker of progression of cervical precancer?” Funded by the Department of Biotechnology, New Delhi. India” from Aug 2006 to July 2009

 

PUBLICATIONS BY THE DEPARTMENT

 

1.      Adurthi S, Sahoo TP, Kumar Chakka, Radhika B, Appaji L, Bapsy PP, Ramesh C and Jayshree RS. Acute toxoplasmosis in nonstem cell transplant patients with haematological malignancies: a study from a Regional Cancer Institute in South India. Hematol Oncol 2008; 26: 229-233.

 

2.      Adurthi S, Krishna S, Mukherjee G, Bafna U D, Uma Devi, Jayshree RS. Regulatory T Cells in a Spectrum of HPV-Induced Cervical Lesions: Cervicitis, Cervical Intraepithelial Neoplasia and Squamous Cell Carcinoma. Am J Reprod Immunol 2008; 60: 55-65.

 

3.      Jayshree RS,  Shafiulla M,  George J,  David JK, Bapsy PP, Chakrabarti A Microscopic, cultural and molecular evidence of disseminated Invasive Aspergillosis involving the lungs and the gastrointestinal tract J Med Microbiol 2006; 55: 961-4

 

4.      Mohammed Shafiulla, Sahoo TP, Jayshree RS, Bapsy PP, Hema Sridhar. " Sino-Oral Zygomycosis Due To Absidia corymbifera In A Patient With Acute Leukemia. Medical Mycology 2004; 42: 475-/8.

 

5.      Human Papillomavirus and oral cancer: the international agency for research on cancer multicenter study. J Natl Cancer Inst 2003; 95:1772 - 1783.

 

6.      Jayshree R.S., Hema Sridhar and Gayatri Devi. Surface, Core and X genes of Hepatitis B Virus in Hepatocellular Carcinoma – An insitu Hybridisation study. Cancer Cytopathology 2003; 99: 63-7.  

 

7.      T. Rajkumar, H. Sridhar, P. Balaram, et al: Oral cancer in Southern India : the influence of body size, diet, infections and sexual practices.  Eur J Cancer Prev 2003; 12: 135-43.

 

8.      Hema Sridhar, Jayshree RS Bapsy PP Appaji L Navin Kumar M, Shafiulla Mohamed VijayKumar BR  Invasive Aspergillosis in Cancer. Mycoses 2002; 45:358-363.

 

9.      Prabha Balaram, Hema Sridhar, Thangarajan Rajkumar, et. al.., - Oral Cancer in Southern India: The influence of Smoking, Drinking, Paan-chewing and Oral hygiene. Int J Cancer 2002; 98: 440-445.

 

10.  Mehta RH, Sridhar H, Vijay Kumar BR, Anand Kumar TC. High incidence of oligozoospermia and teratozoospermia in human semen infected with the aerobic bacterium Streptococcus faecalis. Reprod Biomed Online  2002; 5:17-21.

 

11.  Jayshree R.S., Hema Sridhar, Babu.G, and Suresh T.M. Strongyloides stercoralis eggs in the stools of a patient on anticancer therapy. I J Gastroenterology. 2001; 20:160-161.

 

12.  Jayshree R.S., & Hema Sridhar. p53 Tumor Suppressor Gene Mutations in Hepatocellular Carcinoma Patients in India. Cancer. 2000; 89: 2322-2323.

 

13.  Hema Sridhar, Jayshree R.S., Appaji L, Shafiulla M, & Vijaykumar BR. Fusarium solani fungemia in a patient with acute lymphoid leukemia. Indian J Cancer 2000; 38: 19-21.

 

14.  Poonam.P, Jayshree.R.S, Acharya.A, Hema Sridhar, Babu.G, Suresh T.M. Fulminant fatal Strongyloides stercoralis infection in a post chemotherapy immunosuppressed cancer patient. Medical & Pediatric Oncology 1999; 33: 504 -5.

 

15.  Ravi Kumar R, Mohammed Shafiulla and Hema Sridhar. Association  of   Tuberculosis with malignancy at KIMIO -  An  Oncology Centre, Indian J Pathol  Microbiol 1999; 42: 339 -343.

 

16.  Ain R, Rao J, Peter AT, Vijaykumar BR, Sridhar H, Satish KS, Seshagiri PB. Bacterial infection and endotoxin in female reproductive tract in rats: correlation with the developmental status of preimplantation embryos. Indian J Exp Biol 1998; 36: 867-74.

 

17.  Jayshree.R.S., Acharya. R., & Hema Sridhar. Cyclospora cayetanensis diarrhoea in a patient with Acute Myeloid Leukemia. J Diarrhoeal Dis Res 1998; 16: 254-255.

 

18.  Ravi Kumar R, Vijay Kumar B.R., Mohammed Shafiulla,  Lakshmaiah K.C., and    

      Hema Sridhar.,  Fusarium Solanii infection in a patient with Acute Myelogeneous

      Leukaemia -A     Case Report. Indian J Pathol Microbiol  1997; 40: 555- 557.

 

19.  Jayshree.R.S, Babu G, and Hema Sridhar. Immature Hymenolepis nana worms in the stools of a patient treated for acute lymphoblastic leukemia: an uncommon observation. J Diarrhoeal Dis Res 1997; 15: 75-76.

 

20.  Jayshree R.S., Vijay Kumar & Hema Sridhar. Intestinal parasitic infections in patients with malignancy. J Diarrhoeal Dis Res 1997; 15: 71-74.

 

21.  Sreedharan A., Jayshree R.S., & Hema Sridhar. Cryptosporidiosis among cancer patients: An observation. J Diarrhoeal Dis Res 1996; 14: 211-213.

 

22.  Ravi Kumar.R., Jayshree R.S., & Hema Sridhar. Prevalence of HIV infection in voluntary blood donors & cancer patients. Indian J Pathol Microbiol 1996; 39: 187-190. 

 

23.  Jayshree R. S., Acharya R., & Hema Sridhar. Isospora belli infection in a patient with acute lymphoblastic leukemia in India. J Diarrhoeal Dis Res.  1996; 14:  44-45.

 

24.  Mehta A, Rodrigues C, Kumar R, Rattan A, Sridhar H, Mattoo V, Ginde V. A pilot programme of MRSA surveillance in India. (MRSA Surveillance Study Group). J Postgrad Med. 1996; 42: 1-3.

 

25.  Sreedharan A., Jayshree R.S., Desai A., Hema Sridhar, Chandramukhi A., & Ravi V. Assessment of suitability & ease of performance of seven commercial assay systems for the detection of antibodies to HIV-1.Indian J Med Res 1995; 101: 179-182.

 

26.  Jayshree R.S., Appaji L., & Hema Sridhar. Pneumocystis carinii pneumonia in two patients with Hodgkin's Disease. Indian J Cancer. 1995; 32: 74-76.

 

27.  Usha Niranjan, Hema Sridhar and N. Lalitha- Case report of Bacillus Septicemia:  A rare isolate. Indian J Med Sci 1986; 40: 179-180.